Inbred maize line PHO2T

ABSTRACT

An inbred maize line, designated PH02T, the plants and seeds of inbred maize line PH02T, methods for producing a maize plant produced by crossing the inbred line PH02T with itself or with another maize plant, and hybrid maize seeds and plants produced by crossing the inbred line PH02T with another maize line or plant.

FIELD OF THE INVENTION

This invention is in the field of maize breeding, specifically relatingto an inbred maize line designated PH02T.

BACKGROUND OF THE INVENTION

The goal of plant breeding is to combine in a single variety or hybridvarious desirable traits. For field crops, these traits may includeresistance to diseases and insects, tolerance to heat and drought,reducing the time to crop maturity, greater yield, and better agronomicquality. With mechanical harvesting of many crops, uniformity of plantcharacteristics such as germination and stand establishment, growthrate, maturity, and plant and ear height, is important.

Field crops are bred through techniques that take advantage of theplant's method of pollination. A plant is self-pollinated if pollen fromone flower is transferred to the same or another flower of the sameplant. A plant is cross-pollinated if the pollen comes from a flower ona different plant.

Plants that have been self-pollinated and selected for type for manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny. A cross between twodifferent homozygous lines produces a uniform population of hybridplants that may be heterozygous for many gene loci. A cross of twoplants each heterozygous at a number of gene loci will produce apopulation of hybrid plants that differ genetically and will not beuniform.

Maize (zea mays L.), often referred to as corn in the United States, canbe bred by both self-pollination and cross-pollination techniques. Maizehas separate male and female flowers on the same plant, located on thetassel and the ear, respectively. Natural pollination occurs in maizewhen wind blows pollen from the tassels to the silks that protrude fromthe tops of the ears.

A reliable method of controlling male fertility in plants offers theopportunity for improved plant breeding. This is especially true fordevelopment of maize hybrids, which relies upon some sort of malesterility system. There are several options for controlling malefertility available to breeders, such as: manual or mechanicalemasculation (or detasseling), cytoplasmic male sterility, genetic malesterility, gametocides and the like.

Hybrid maize seed is typically produced by a male sterility systemincorporating manual or mechanical detasseling. Alternate strips of twomaize inbreds are planted in a field, and the pollen-bearing tassels areremoved from one of the inbreds (female). Providing that there issufficient isolation from sources of foreign maize pollen, the ears ofthe detasseled inbred will be fertilized only from the other inbred(male), and the resulting seed is therefore hybrid and will form hybridplants.

The laborious, and occasionally unreliable, detasseling process can beavoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMSinbred are male sterile as a result of factors resulting from thecytoplasmic, as opposed to the nuclear, genome. Thus, thischaracteristic is inherited exclusively through the female parent inmaize plants, since only the female provides cytoplasm to the fertilizedseed. CMS plants are fertilized with pollen from another inbred that isnot male-sterile. Pollen from the second inbred may or may notcontribute genes that make the hybrid plants male-fertile. Seed fromdetasseled fertile maize and CMS produced seed of the same hybrid can beblended to insure that adequate pollen loads are available forfertilization when the hybrid plants are grown.

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations asdescribed by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. Theseand all patents referred to are incorporated by reference. In additionto these methods, Albertsen et al., of Pioneer Hi-Bred, U.S. patentapplication Ser. No. 07/848,433, have developed a system of nuclear malesterility which includes: identifying a gene which is critical to malefertility; silencing this native gene which is critical to malefertility; removing the native promoter from the essential malefertility gene and replacing it with an inducible promoter; insertingthis genetically engineered gene back into the plant; and thus creatinga plant that is male sterile because the inducible promoter is not "on"resulting in the male fertility gene not being transcribed. Fertility isrestored by inducing, or turning "on", the promoter, which in turnallows the gene that confers male fertility to be transcribed.

There are many other methods of conferring genetic male sterility in theart, each with its own benefits and drawbacks. These methods use avariety of approaches such as delivering into the plant a gene encodinga cytotoxic substance associated with a male tissue specific promoter oran antisense system in which a gene critical to fertility is identifiedand an antisense to that gene is inserted in the plant (see:Fabinjanski, et al. EPO 89/3010153.8 publication no. 329,308 and PCTapplication PCT/CA90/00037 published as WO 90/08828).

Another system useful in controlling male sterility makes use ofgametocides. Gametocides are not a genetic system, but rather a topicalapplication of chemicals. These chemicals affect cells that are criticalto male fertility. The application of these chemicals affects fertilityin the plants only for the growing season in which the gametocide isapplied (see Carlson, Glenn R., U.S. Pat. No. 4,936,904). Application ofthe gametocide, timing of the application and genotype specificity oftenlimit the usefulness of the approach.

The use of male sterile inbreds is but one factor in the production ofmaize hybrids. The development of maize hybrids requires, in general,the development of homozygous inbred lines, the crossing of these lines,and the evaluation of the crosses. Pedigree breeding and recurrentselection breeding methods are used to develop inbred lines frombreeding populations. Breeding programs combine the genetic backgroundsfrom two or more inbred lines or various other germplasm sources intobreeding pools from which new inbred lines are developed by selfing andselection of desired phenotypes. The new inbreds are crossed with otherinbred lines and the hybrids from these crosses are evaluated todetermine which of those have commercial potential. Plant breeding andhybrid development are expensive and time consuming processes.

Pedigree breeding starts with the crossing of two genotypes, each ofwhich may have one or more desirable characteristics that is lacking inthe other or which complements the other. If the two original parents donot provide all the desired characteristics, other sources can beincluded in the breeding population. In the pedigree method, superiorplants are selfed and selected in successive generations. In thesucceeding generations the heterozygous condition gives way tohomogeneous lines as a result of self-pollination and selection.Typically in the pedigree method of breeding five or more generations ofselfing and selection is practiced: F₁ →F₂ ; F₃ →F₄ ; F₄ →F₅, etc.

Recurrent selection breeding, backcrossing for example, can be used toimprove an inbred line. Backcrossing can be used to transfer a specificdesirable trait from one inbred or source to an inbred that lacks thattrait. This can be accomplished, for example, by first crossing asuperior inbred (recurrent parent) to a donor inbred (non-recurrentparent), that carries the appropriate gene(s) for the trait in question.The progeny of this cross is then mated back to the superior recurrentparent followed by selection in the resultant progeny for the desiredtrait to be transferred from the non-recurrent parent. After five ormore backcross generations with selection for the desired trait, theprogeny will be heterozygous for loci controlling the characteristicbeing transferred, but will be like the superior parent for most oralmost all other genes. The last backcross generation is then selfed togive pure breeding progeny for the gene(s) being transferred.

A single cross maize hybrid results from the cross of two inbred lines,each of which has a genotype that complements the genotype of the other.The hybrid progeny of the first generation is designated F₁. In thedevelopment of commercial hybrids only the F₁ hybrid plants are sought.Preferred F₁ hybrids are more vigorous than their inbred parents. Thishybrid vigor, or heterosis, can be manifested in many polygenic traits,including increased vegetative growth and increased yield.

The development of a maize hybrid involves three steps: (1) theselection of plants from various germplasm pools for initial breedingcrosses; (2) the selfing of the selected plants from the breedingcrosses for several generations to produce a series of inbred lines,which, although different from each other, breed true and are highlyuniform; and (3) crossing the selected inbred lines with differentinbred lines to produce the hybrid progeny (F₁). During the inbreedingprocess in maize, the vigor of the lines decreases. Vigor is restoredwhen two different inbred lines are crossed to produce the hybridprogeny (F₁). An important consequence of the homozygosity andhomogeneity of the inbred lines is that the hybrid between a definedpair of inbreds will always be the same. Once the inbreds that give asuperior hybrid have been identified, the hybrid seed can be reproducedindefinitely as long as the homogeneity of the inbred parents ismaintained.

A single cross hybrid is produced when two inbred lines are crossed toproduce the F₁ progeny. A double cross hybrid is produced from fourinbred lines crossed in pairs (A×B and C×D) and then the two F₁ hybridsare crossed again (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁hybrids is lost in the next generation (F₂). Consequently, seed fromhybrids is not used for planting stock.

Hybrid seed production requires elimination or inactivation of pollenproduced by the female parent. Incomplete removal or inactivation of thepollen provides the potential for self pollination. This inadvertentlyself pollinated seed may be unintentionally harvested and packaged withhybrid seed.

Once the seed is planted, it is possible to identify and select theseself pollinated plants. These self pollinated plants will be geneticallyequivalent to the female inbred line used to produce the hybrid.

Typically these self pollinated plants can be identified and selecteddue to their decreased vigor. Female selfs are identified by their lessvigorous appearance for vegetative and/or reproductive characteristics,including shorter plant height, small ear size, ear and kernel shape,cob color, or other characteristics.

Identification of these self pollinated lines can also be accomplishedthrough molecular marker analyses. See, "The Identification of FemaleSelfs in Hybrid Maize: A Comparison Using Electrophoresis andMorphology", Smith, J. S. C. and Wych, R. D., Seed Science andTechnology 14, pp. 1-8 (1995), the disclosure of which is expresslyincorporated herein by reference. Through these technologies, thehomozygosity of the self pollinated line can be verified by analyzingallelic composition at various loci along the genome. Those methodsallow for rapid identification of the invention disclosed herein. Seealso, "Identification of Atypical Plants in Hybrid Maize Seed byPostcontrol and Electrophoresis" Sarca, V. et al., Probleme de GeneticaTeoritica si Aplicata Vol. 20 (1) p. 29-42.

As is readily apparent to one skilled in the art, the foregoing are onlytwo of the various ways by which the inbred can be obtained by thoselooking to use the germplasm. Other means are available, and the aboveexamples are illustrative only

Maize is an important and valuable field crop. Thus, a continuing goalof plant breeders is to develop high-yielding maize hybrids that areagronomically sound based on stable inbred lines. The reasons for thisgoal are obvious: to maximize the amount of grain produced with theinputs used and minimize susceptibility of the crop to pests andenvironmental stresses. To accomplish this goal, the maize breeder mustselect and develop superior inbred parental lines for producing hybrids.This requires identification and selection of genetically uniqueindividuals that occur in a segregating population. The segregatingpopulation is the result of a combination of crossover events plus theindependent assortment of specific combinations of alleles at many geneloci that results in specific genotypes. The probability of selectingany one individual with a specific genotype from a breeding cross isinfinitesimal due to the large number of segregating genes and theunlimited recombinations of these genes, some of which may be closelylinked. However, the genetic variation among individual progeny of abreeding cross allows for the identification of rare and valuable newgenotypes. These new genotypes are neither predictable nor incrementalin value, but rather the result of manifested genetic variation combinedwith selection methods, environments and the actions of the breeder.

Thus, even if the entire genotypes of the parents of the breeding crosswere characterized and a desired genotype known, only a few, if any,individuals having the desired genotype may be found in a largesegregating F₂ population. Typically, however, neither the genotypes ofthe breeding cross parents nor the desired genotype to be selected isknown in any detail. In addition, it is not known how the desiredgenotype would react with the environment. This genotype by environmentinteraction is an important, yet unpredictable, factor in plantbreeding. A breeder of ordinary skill in the art cannot predict thegenotype, how that genotype will interact with various climaticconditions or the resulting phenotypes of the developing lines, exceptperhaps in a very broad and general fashion. A breeder of ordinary skillin the art would also be unable to recreate the same line twice from thevery same original parents as the breeder is unable to direct how thegenomes combine or how they will interact with the environmentalconditions. This unpredictability results in the expenditure of largeamounts of research resources in the development of a superior new maizeinbred line.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred maize line,designated PH02T. This invention thus relates to the seeds of inbredmaize line PH02T, to the plants of inbred maize line PH02T, and tomethods for producing a maize plant produced by crossing the inbred linePH02T with itself or another maize line. This invention further relatesto hybrid maize seeds and plants produced by crossing the inbred linePH02T with another maize line.

Definitions

In the description and examples that follow, a number of terms are usedherein. In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided. NOTE: ABS is in absolute termsand % MN is percent of the mean for the experiments in which the inbredor hybrid was grown. These designators will follow the descriptors todenote how the values are to be interpreted. Below are the descriptorsused in the data tables included herein.

ANT ROT=ANTHRACNOSE STALK ROT (Colletotrichum graminicola). A 1 to 9visual rating indicating the resistance to Anthracnose Stalk Rot. Ahigher score indicates a higher resistance.

BAR PLT=BARREN PLANTS. The percent of plants per plot that were notbarren (lack ears).

BRT STK=BRITTLE STALKS. This is a measure of the stalk breakage near thetime of pollination, and is an indication of whether a hybrid or inbredwould snap or break near the time of flowering under severe winds. Dataare presented as percentage of plants that did not snap.

BU ACR=YIELD (BUSHELS/ACRE). Yield of the grain at harvest in bushelsper acre adjusted to 15.5% moisture.

CLD TST=COLD TEST. The percent of plants that germinate under cold testconditions.

CLN=CORN LETHAL NECROSIS. Synergistic interaction of maize chloroticmottle virus (MCMV) in combination with either maize dwarf mosaic virus(MDMV-A or MDMV-B) or wheat streak mosaic virus (WSMV). A 1 to 9 visualrating indicating the resistance to Corn Lethal Necrosis. A higher scoreindicates a higher resistance.

COM RST=COMMON RUST (Puccinia sorghi). A 1 to 9 visual rating indicatingthe resistance to Common Rust. A higher score indicates a higherresistance.

D/D=DRYDOWN. This represents the relative rate at which a hybrid willreach acceptable harvest moisture compared to other hybrids on a 1-9rating scale. A high score indicates a hybrid that dries relatively fastwhile a low score indicates a hybrid that dries slowly.

DIP ERS=DIPLODIA EAR MOLD SCORES (Diplodia maydis and Diplodiamacrospora). A 1 to 9 visual rating indicating the resistance toDiplodia Ear Mold. A higher score indicates a higher resistance.

DRP EAR=DROPPED EARS. A measure of the number of dropped ears per plotand represents the percentage of plants that did not drop ears prior toharvest.

DIT=DROUGHT TOLERANCE. This represents a 1-9 rating for droughttolerance, and is based on data obtained under stress conditions. A highscore indicates good drought tolerance and a low score indicates poordrought tolerance.

EAR HT=EAR HEIGHT. The ear height is a measure from the ground to thehighest placed developed ear node attachment and is measured in inches.

EAR MLD=General Ear Mold. Visual rating (1-9 score) where a "1" is verysusceptible and a "9" is very resistant. This is based on overall ratingfor ear mold of mature ears without determining the specific moldorganism, and may not be predictive for a specific ear mold.

EAR SZ=EAR SIZE. A 1 to 9 visual rating of ear size. The higher therating the larger the ear size.

ECB 1LF=EUROPEAN CORN BORER FIRST GENERATION LEAF FEEDING (Ostrinianubilalis). A 1 to 9 visual rating indicating the resistance topreflowering leaf feeding by first generation European Corn Borer. Ahigher score indicates a higher resistance.

ECB 2IT=EUROPEAN CORN BORER SECOND GENERATION INCHES OF TUNNELING(Ostinia nubilalis). Average inches of tunneling per plant in the stalk.

ECB 2SC=EUROPEAN CORN BORER SECOND GENERATION (Ostrinia nubilalis). A 1to 9 visual rating indicating post flowering degree of stalk breakageand other evidence of feeding by European Corn Borer, Second Generation.A higher score indicates a higher resistance.

ECB DPE=EUROPEAN CORN BORER DROPPED EARS (Ostrinia nubilalis). Droppedears due to European Corn Borer. Percentage of plants that did not dropears under second generation corn borer infestation.

EST CNT=EARLY STAND COUNT. This is a measure of the stand establishmentin the spring and represents the number of plants that emerge on perplot basis for the inbred or hybrid.

EYE SPT=Eye Spot (Kabatiella zeae or Aureobasidium zeae). A 1 to 9visual rating indicating the resistance to Eye Spot. A higher scoreindicates a higher resistance.

FUS ERS=FUSARIUM EAR ROT SCORE (Fusarium moniliforme or Fusariumsubglutinans). A 1 to 9 visual rating indicating the resistance toFusarium ear rot. A higher score indicates a higher resistance.

GDU=Growing Degree Units. Using the Barger Heat Unit Theory, whichassumes that maize growth occurs in the temperature range 50° F.-86° F.and that temperatures outside this range slow down growth; the maximumdaily heat unit accumulation is 36 and the minimum daily heat unitaccumulation is 0. The seasonal accumulation of GDU is a major factor indetermining maturity zones.

GDU SHD=GDU TO SHED. The number of growing degree units (GDUs) or heatunits required for an inbred line or hybrid to have approximately 50percent of the plants shedding pollen and is measured from the time ofplanting. Growing degree units are calculated by the Barger Method,where the heat units for a 24-hour period are: ##EQU1##

The highest maximum temperature used is 86° F. and the lowest minimumtemperature used is 50° F. For each inbred or hybrid it takes a certainnumber of GDUs to reach various stages of plant development.

GDU SLK=GDU TO SILK. The number of growing degree units required for aninbred line or hybrid to have approximately 50 percent of the plantswith silk emergence from time of planting. Growing degree units arecalculated by the Barger Method as given in GDU SHD definition.

GIB ERS=GIBBERELLA EAR ROT (PINK MOLD) (Gibberella zeae). A 1 to 9visual rating indicating the resistance to Gibberella Ear Rot. A higherscore indicates a higher resistance.

GLF SPT=Gray Leaf Spot (Cercospora zeae-maydis). A 1 to 9 visual ratingindicating the resistance to Gray Leaf Spot. A higher score indicates ahigher resistance.

GOS WLT=Goss' Wilt (Corynebacterium nebraskense). A 1 to 9 visual ratingindicating the resistance to Goss' Wilt. A higher score indicates ahigher resistance.

GRN APP=GRAIN APPEARANCE. This is a 1 to 9 rating for the generalappearance of the shelled grain as it is harvested based on such factorsas the color of harvested grain, any mold on the grain, and any crackedgrain. High scores indicate good grain quality.

H/POP=YIELD AT HIGH DENSITY. Yield ability at relatively high plantdensities on 1-9 relative rating system with a higher number indicatingthe hybrid responds well to high plant densities for yield relative toother hybrids. A 1, 5, and 9 would represent very poor, average, andvery good yield response, respectively, to increased plant density.

HC BLT=HELMINTHOSPORIUM CARBONUM LEAF BLIGHT (Helminthosporiumcarbonum). A 1 to 9 visual rating indicating the resistance toHelminthosporium infection. A higher score indicates a higherresistance.

HD SMT=HEAD SMUT (Sphacelotheca reiliana). This score indicates thepercentage of plants not infected.

INC D/A=GROSS INCOME (DOLLARS PER ACRE). Relative income per acreassuming drying costs of two cents per point above 15.5 percent harvestmoisture and current market price per bushel.

INCOME/ACRE. Income advantage of hybrid to be patented over other hybridon per acre basis.

INC ADV=GROSS INCOME ADVANTAGE. GROSS INCOME advantage of variety #1over variety #2.

KSZ DCD=KERNEL SIZE DISCARD. The percent of discard seed; calculated asthe sum of discarded tip kernels and extra large kernels.

UPOP=YIELD AT LOW DENSITY. Yield ability at relatively low plantdensities on a 1-9 relative system with a higher number indicating thehybrid responds well to low plant densities for yield relative to otherhybrids. A 1, 5, and 9 would represent very poor, average, and very goodyield response, respectively, to low plant density.

MDM CPX=MAIZE DWARF MOSAIC COMPLEX (MDMV=Maize Dwarf Mosaic Virus andMCDV=Maize Chlorotic Dwarf Virus). A 1 to 9 visual rating indicating theresistance to Maize Dwarf Mosaic Complex. A higher score indicates ahigher resistance.

MST=HARVEST MOISTURE. The moisture is the actual percentage moisture ofthe grain at harvest.

MST ADV=MOISTURE ADVANTAGE. The moisture advantage of variety #1 overvariety #2 as calculated by: MOISTURE of variety #2-MOISTURE of variety#1=MOISTURE ADVANTAGE of variety #1.

NLF BLT=Northern Leaf Blight (Helminthosporium turcicum or Exserohilumturcicum). A 1 to 9 visual rating indicating the resistance to NorthernLeaf Blight. A higher score indicates a higher resistance.

PLT HT=PLANT HEIGHT. This is a measure of the height of the plant fromthe ground to the tip of the tassel in inches.

POL SC=POLLEN SCORE. A 1 to 9 visual rating indicating the amount ofpollen shed. The higher the score the more pollen shed.

POL WT=POLLEN WEIGHT. This is calculated by dry weight of tasselscollected as shedding commences minus dry weight from similar tasselsharvested after shedding is complete.

It should be understood that the inbred can, through routinemanipulation of cytoplasmic or other factors, be produced in amale-sterile form. Such embodiments are also contemplated within thescope of the present claims.

POP K/A=PLANT POPULATIONS. Measured as 1000s per acre.

POP ADV=PLANT POPULATION ADVANTAGE. The plant population advantage ofvariety #1 over variety #2 as calculated by PLANT POPULATION of variety#2-PLANT POPULATION of variety #1=PLANT POPULATION ADVANTAGE of variety#1.

PRM=PREDICTED RELATIVE MATURITY. This trait, predicted relativematurity, is based on the harvest moisture of the grain. The relativematurity rating is based on a known set of checks and utilizes standardlinear regression analyses and is also referred to as the ComparativeRelative Maturity Rating System that is similar to the MinnesotaRelative Maturity Rating System.

PRM SHD=A relative measure of the growing degree units (GDU) required toreach 50% pollen shed. Relative values are predicted values from thelinear regression of observed GDU's on relative maturity of commercialchecks.

RT LDG=ROOT LODGING. Root lodging is the percentage of plants that donot root lodge; plants that lean from the vertical axis at anapproximately 30° angle or greater would be counted as root lodged.

RTL ADV=ROOT LODGING ADVANTAGE. The root lodging advantage of variety #1over variety #2.

SCT GRN=SCATTER GRAIN. A 1 to 9 visual rating indicating the amount ofscatter grain (lack of pollination or kernel abortion) on the ear. Thehigher the score the less scatter grain.

SDG VGR=SEEDLING VIGOR. This is the visual rating (1 to 9) of the amountof vegetative growth after emergence at the seedling stage(approximately five leaves). A higher score indicates better vigor.

SEL IND=SELECTION INDEX. The selection index gives a single measure ofthe hybrid's worth based on information for up to five traits. A maizebreeder may utilize his or her own set of traits for the selectionindex. One of the traits that is almost always included is yield. Theselection index data presented in the tables represent the mean valueaveraged across testing stations.

SLF BLT=SOUTHERN LEAF BLIGHT (Helminthosporium maydis or Bipolarismaydis). A 1 to 9 visual rating indicating the resistance to SouthernLeaf Blight. A higher score indicates a higher resistance.

SOU RST=SOUTHERN RUST (Puccinia polysora). A 1 to 9 visual ratingindicating the resistance to Southern Rust. A higher score indicates ahigher resistance.

STA GRN=STAY GREEN. Stay green is the measure of plant health near thetime of black layer formation (physiological maturity). A high scoreindicates better late-season plant health.

STD ADV=STALK STANDING ADVANTAGE. The advantage of variety #1 overvariety #2 for the trait STK CNT.

STK CNT=NUMBER OF PLANTS. This is the final stand or number of plantsper plot.

STK LDG=STALK LODGING. This is the percentage of plants that did notstalk lodge (stalk breakage) as measured by either natural lodging orpushing the stalks and determining the percentage of plants that breakbelow the ear.

STW WLT=Stewart's Wilt (Erwinia stewartii). A 1 to 9 visual ratingindicating the resistance to Stewart's Wilt. A higher score indicates ahigher resistance.

TAS BLS=TASSEL BLAST. A 1 to 9 visual rating was used to measure thedegree of blasting (necrosis due to heat stress) of the tassel at thetime of flowering. A 1 would indicate a very high level of blasting attime of flowering, while a 9 would have no tassel blasting.

TAS SZ=TASSEL SIZE. A 1 to 9 visual rating was used to indicate therelative size of the tassel. The higher the rating the larger thetassel.

TAS WT=TASSEL WEIGHT. This is the average weight of a tassel (grams)just prior to pollen shed.

TEX EAR=EAR TEXTURE. A 1 to 9 visual rating was used to indicate therelative hardness (smoothness of crown) of mature grain. A 1 would bevery soft (extreme dent) while a 9 would be very hard (flinty or verysmooth crown).

TILLER=TILLERS. A count of the number of tillers per plot that couldpossibly shed pollen was taken. Data are given as a percentage oftillers: number of tillers per plot divided by number of plants perplot.

TST WT=TEST WEIGHT (UNADJUSTED). The measure of the weight of the grainin pounds for a given volume (bushel).

TST WTA=TEST WEIGHT ADJUSTED. The measure of the weight of the grain inpounds for a given volume (bushel) adjusted for 15.5 percent moisture.

TSW ADV=TEST WEIGHT ADVANTAGE. The test weight advantage of variety #1over variety #2.

WIN M %=PERCENT MOISTURE WINS.

WIN Y %=PERCENT YIELD WINS.

YLD=YIELD. It is the same as BU ACR ABS.

YLD ADV=YIELD ADVANTAGE. The yield advantage of variety #1 over variety#2 as calculated by: YIELD of variety #1-YIELD variety #2=yieldadvantage of variety #1.

YLD SC=YIELD SCORE. A 1 to 9 visual rating was used to give a relativerating for yield based on plot ear piles. The higher the rating thegreater visual yield appearance.

DETAILED DESCRIPTION OF THE INVENTION

Inbred maize lines are typically developed for use in the production ofhybrid maize lines. Inbred maize lines need to be highly homogeneous,homozygous and reproducible to be useful as parents of commercialhybrids.There are many analytical methods available to determine thehomozygotic and phenotypic stability of these inbred lines.

The oldest and most traditional method of analysis is the observation ofphenotypic traits. The data is usually collected in field experimentsoverthe life of the maize plants to be examined. Phenotypiccharacteristics most often observed are for traits associated with plantmorphology, ear and kernel morphology, insect and disease resistance,maturity, and yield.

In addition to phenotypic observations, the genotype of a plant can alsobeexamined. There are many laboratory-based techniques available for theanalysis, comparison and characterization of plant genotype; among theseare Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms(RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily PrimedPolymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting(DAF), Sequence Characterized Amplified Regions (SCARs), AmplifiedFragment Length Polymorphisms (AFLPs), and Simple Sequence Repeats(SSRs) which are also referred to as Microsatellites.

The most widely used of these laboratory techniques are IsozymeElectrophoresis and RFLPs as discussed in Lee, M., "Inbred Lines ofMaize and Their Molecular Markers," The Maize Handbook,(Springer-Verlag, New York, Inc. 1994, at 423-432) incorporated hereinby reference. Isozyme Electrophoresis is a useful tool in determininggenetic composition, although it has relatively low number of availablemarkers and the low number of allelic variants among maize inbreds.RFLPs have the advantage of revealing an exceptionally high degree ofallelic variation in maize and the number of available markers is almostlimitless.

Maize RFLP linkage maps have been rapidly constructed and widelyimplemented in genetic studies. One such study is described inBoppenmaier, et al., "Comparisons among strains of inbreds for RFLPs",Maize Genetics Cooperative Newsletter, 65: 1991, pg. 90, is incorporatedherein by reference. This study used 101 RFLP markers to analyze thepatterns of 2 to 3 different deposits each of five different inbredlines.The inbred lines had been selfed from 9 to 12 times before beingadopted into 2 to 3 different breeding programs. It was results fromthese 2 to 3 different breeding programs that supplied the differentdeposits for analysis. These five lines were maintained in the separatebreeding programs by selfing or sibbing and rogueing off-type plants foran additional one to eight generations. After the RFLP analysis wascompleted, it was determined the five lines showed 0-2% residualheterozygosity. Although this was a relatively small study, it can beseenusing RFLPs that the lines had been highly homozygous prior to theseparatestrain maintenance.

Inbred maize line PH02T is a yellow, dent maize inbred that is bestsuited as a male for producing first generation F₁ maize hybrids, butcan beused as a female. Inbred maize line PH02T is best adapted to theCentral Corn Belt, Southeast and Southcentral regions of the UnitedStates and canbe used to produce hybrids from approximately 118 relativematurity based on the Comparative Relative Maturity Rating System forharvest moisture ofgrain. Inbred maize line PH02T demonstrates goodyield, good stay green andexcellent pollen shed. In hybrid combination,PH02T demonstrates high, stable yields, excellent resistance to GrayLeaf Spot, excellent stay green, very good stalk strength and high testweight grain. For its area of adaptation, PH02T demonstrates high,stable yields, excellent resistance to Gray Leaf Spot, very good staygreen, superior stalk strength and high test weight grain.

The inbred has shown uniformity and stability within the limits ofenvironmental influence for all the traits as described in the VarietyDescription Information (Table 1) that follows. The inbred has beenself-pollinated and ear-rowed a sufficient number of generations withcareful attention paid to uniformity of plant type to ensure thehomozygosity and phenotypic stability necessary to use in commercialproduction. The line has been increased both by hand and in isolatedfields with continued observation for uniformity. No variant traits havebeen observed or are expected in PH02T.

Inbred maize line PH02T, being substantially homozygous, can bereproduced by planting seeds of the line, growing the resulting maizeplants under self-pollinating or sib-pollinating conditions withadequate isolation, and harvesting the resulting seed, using techniquesfamiliar to the agricultural arts.

                                      TABLE 1    __________________________________________________________________________    VARIETY DESCRIPTION INFORMATION    VARIETY = PH02T    __________________________________________________________________________      TYPE: (describe intermediate types in Comments section):    2     1 = Sweet 2 = Dent 3 = Flint 4 = Flour 5 = Pop 6 = Ornamental      MATURITY:    DAYS  HEAT UNITS    076   1,537.5                 From emergence to 50% of plants in silk    077   1,561.3                 From emergence to 50% of plants in pollen    005   0,119.8                 From 10% to 90% pollen shed    067   1,163.5                 From 50% silk to harvest at 25% moisture      PLANT:                                       Standard                                            Sample                                       Deviation                                            Size    0,221.3          cm     Plant Height (to tassel tip)                                       8.22 4    0,084.0          cm     Ear Height (to base of top ear node)                                       4.32 4    0,016.4          cm     Length of Top Ear Intermode                                       1.17 20    1            Average Number of Tillers                                       0.00 4    1.0          Average Number of Ears per Stalk                                       0.00 4    3.0          Anthocyanin of Brace Roots: 1 = Absent 2 = Faint 3 =                 Moderate 4 = Dark      LEAF:                                       Standard                                            Sample                                       Deviation                                            Size    010.4 cm     Width of Ear Node Leaf                                       1.00 20    082.2 cm     Length of Ear Node Leaf                                       2.08 20    06.1         Number of leaves above top ear                                       0.34 20    030.0        Degrees Leaf Angle (measure from 2nd leaf above                                       12.99                                            4                 ear at anthesis to stalk above leaf)    03           Leaf Color                       Dark Green                             (Munsell code) 5GY44    1.0          Leaf Sheath Pubescence (Rate on scale from 1 = none to 9 =                 like peach fuzz)    5.7          Marginal Waves (Rate on scale from 1 = none to 9 = many)    6.0          Longitudinal Creases (Rate on scale from 1 = none to 9 =                 many)      TASSEL:                                       Standard                                            Sample                                       Deviation                                            Size    08.8         Number of Primary Lateral Branches                                       2.35 20    046.7        Branch Angle from Central Spike                                       16.26                                            3    52.5  cm     Tassel Length (from top leaf collar to tassel                                       2.22 4    7.8          Pollen Shed (rate on scale from 0 = male sterile to 9 =                 heavy shed)    11           Anther Color                       Pink  (Munsell code) 10RP48    01           Glume Color                       Light Green                             (Munsell code) 5GY58    1.0          Bar Glumes (Glume Bands): 1 = Absent 2 = Present    16           Peduncle Length (cm. from top leaf to basal branches)    6a.      EAR (Unhusked Data):    9     Silk Color (3 days after emergence)                              Salmon                                    (Munsell code)                                                7.5Y74    1     Fresh Husk Color (25 days after 50% silking)                              Light Green                                    (Munsell code)                                                5GY68    21    Dry Husk Color (65 days after 50% silking)                              Buff  (Munsell code)                                                2.5y8.54    Position of Ear at Dry Husk Stage: 1 = Upright 2 = Horizontal 3 =    Pendant    5     Husk Tightness (Rate of Scale From 1 = very loose to 9 = very          tight)    2     Husk Extenson (at harvest): 1 = Short (ears exposed) 2 = Medium (<8          cm)    3 = Long (8-10 cm beyond ear tip) 4 = Very Long (>10 cm)                                                Medium    6b.      EAR (Husked Ear Data):                                       Standard                                            Sample                                       Deviation                                            Size    17    cm     Ear Length            0.41 20    41    mm     Ear Diameter at mid-point                                       2.60 20    132   gm     Ear Weight            20.24                                            20    15           Number of Kernel Rows 0.86 20    2            Kernel Rows: 1 = Indistinct 2 = Distinct                                                Distinct    1            Row Alignment: 1 = Straight 2 = Slightly Curved 3                                                Straight    11    cm     Shank Length          1.47 20                 Ear Taper: 1 = Slight 2 = Average 3 = Extreme                                                Average    2            Ear Taper: 1 = Slight 2 = Average 3 = Extreme                                                Average      KERNEL (Dried):                                       Standard                                            Sample                                       Deviation                                            Size    11    mm     Kernel Length         0.98 20    8     mm     Kernel Width          0.49 20    5     mm     Kernel Thickness      0.26 20    20           % Round Kernels (Shape Grade)                                       6.59 4    1            Aleurone Color Pattern: 1 = Homozygous 2 = Segregating                                                Homozygous    7            Aleurone Color                              Yellow   (Munsell code)                                                10YR714    7            Hard Endosperm Color                              Yellow   (Munsell code)                                                10YR714    3            Endosperm Type:                              Normal Starch                 1 = Sweet (Su1) 2 = Extra Sweet (sh2) 3 = Normal Starch                 4 = High Amylose Starch 5 = Waxy Starch 6 = High Protein                 7 = High Lysine 8 = Super Sweet (se) 9 = High Oil                 10 = Other    27    gm     Weight per 100 Kernels (unsized sample)                                       4.69 4      COB:                                       Standard                                            Sample                                       Deviation                                            Size    23    mm     Cob Diameter at mid-point                                       1.87 20    14           Cob Color    Red      (Munsell code)                                                10R58    2            Cob Strength 1 = Weak 2 = Strong      DISEASE RESISTANCE (Rate from 1 (most susceptible) to 9 (most      resistant); leave blank      if not tested; leave Race or Strain Options blank if polygenic):    A.    Leaf Blights, Wilts, and Local Infection Diseases                 Anthracnose Leaf Blight (Colletotrichum graminicola)    6            Common Rust (Puccinia sorghi)                 Common Smut (Ustilago                 maydis) Eyespot (Kabatiella zeae)                 Goss's Wilt (Clavibacter michiganense spp. nebraskense)    6            Gray Leaf Spot (Carcospora zeae-maydis)                 Helminthosporium Leaf Spot (Bipolaris zeicola) Race    4            Northern Leaf Blight (Exserohilum turcicum) Race    5            Southern Leaf Blight (Bipolaris maydis) Race                 Southern Rust (Puccinia polysora)    7            Stewart's Wilt (Erwinia stewartii)                 Other (Specify)    B.    Systemic Diseases    5            Corn Lethal Necrosis (MCMV and MDMV)                 Head Smut (Sphacelotheca reiliana)                 Maize Chlorotic Dwarf Virus (MDV)                 Maize Chlorotic Mottle Virus (MCMV)    4            Maize Dwarf Mosaic Virus (MDMV)                 Sorghum Downy Mildew of Corn (Peronosclerospora sorghi)                 Other (Specify)    C.    Stalk Rots    4            Anthracnose Stalk Rot (Colletotrichum graminicola)                 Diplodia Stalk Rot (Stenocarpelia maydis)                 Fusarium Stalk Rot (Fusarium moniliforme)                 Gibberella Stalk Rot (Gibberella zeae)                 Other (Specify)    D.    Ear and Kernel Rots                 Aspergillus Ear and Kernel Rot (Aspergillus flavus)    2            Diplodia Ear Rot (Stenocarpella maydis)    4            Fusarium Ear and Kernel Rot (Fusarium moniliforme)                 Gibberella Ear Rot (Gibberella zeae)                 Other (Specify)                 Banks grass Mite (Oligonychus pratensis)                 Corn Worm (Helicoverpa zea)                 Leaf Feeding                 Silk Feeding                 mg larval wt.                 Ear Damage                 Corn Leaf Aphid (Rhopalosiphum maidis)                 Corn Sap Beetle (Carpophilus dimidiatus                 European Corn Borer (Ostrinia nubilalis)    4            1st Generation (Typicaly Whorf Leaf Feeding)                 2nd Generation (Typically Leaf Sheath-Collar Feeding)                 Stalk Tunneling                 cm tunneled/plant                 Fall Armyworm (Spodoptera fruqiperda)                 Leaf Feeding                 Silk Feeding                 mg larval wt.                 Maize Weevil (Sitophilus zeamaize                 Northern Rootworm (Diabrotica barberi)                 Southern Rootworm (Diabrotica undecimpunctata)                 Southwestern Corn Borer (Diatreaea grandiosella)                 Leaf Feeding                 Stalk Tunneling                 cm tunneled/plant                 Two-spotted Spider Mite (Tetranychus utricae)                 Western Rootworm (Diabrotica virgifrea virgifera)                 Other (Specify)      AGRONOMIC TRAITS:    7            Staygreen (at 65 days after anthesis) (Rate on a scale from                 1 = worst to                 excellent)    0.0          % Dropped Ears (at 65 days after anthesis)                 % Pre-anthesis Brittle Snapping                 % Pre-anthesis Root Lodging    10.3         Post-anthesis Root Lodging (at 65 days after anthesis)    5,810        Kg/ha Yield (at 12-13% grain moisture)    __________________________________________________________________________    *In interpreting the foregoing color designations, reference may be had to     the Munsell Glossy Book of Color, a standard color reference.

Industrial Applicability

This invention also is directed to methods for producing a maize plantby crossing a first parent maize plant with a second parent maize plantwherein either the first or second parent maize plant is an inbred maizeplant of the line PH02T. Further, both first and second parent maizeplants can come from the inbred maize line PH02T. Thus, any such methodsusing the inbred maize line PH02T are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, and the like.All plants produced using inbred maize line PH02T as a parent are withinthe scope of this invention. Advantageously, the inbred maize line isused in crosses with other, different, maize inbreds to produce firstgeneration (F₁) maize hybrid seeds and plants with superiorcharacteristics.

As used herein, the term plant includes plant cells, plant protoplasts,plant cell tissue cultures from which maize plants can be regenerated,plant calli, plant clumps, and plant cells that are intact in plants orparts of plants, such as embryos, pollen, ovules, flowers, kernels,ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk andthe like.

Duncan, Williams, Zehr, and Widholm, Planta (1985) 165: 322-332 reflectsthat 97% of the plants cultured that produced callus were capable ofplantregeneration. Subsequent experiments with both inbreds and hybridsproduced91% regenerable callus that produced plants. In a further studyin 1988, Songstad, Duncan & Widholm in Plant Cell Reports (1988),7:262-265 reportsseveral media additions that enhance regenerability ofcallus of two inbredlines. Other published reports also indicated that"nontraditional" tissuesare capable of producing somatic embryogenesisand plant regeneration. K. P. Rao, et al., Maize Genetics CooperationNewsletter, 60:64-65 (1986), refers to somatic embryogenesis from glumecallus cultures and B. V. Conger, et al., Plant Cell Reports, 6: 345-347(1987) indicates somatic embryogenesis from the tissue cultures of maizeleaf segments. Thus, it isclear from the literature that the state ofthe art is such that these methods of obtaining plants are, and were,"conventional" in the sense that they are routinely used and have a veryhigh rate of success.

Tissue culture of maize is described in European Patent Application,publication 160,390, incorporated herein by reference. Maize tissueculture procedures are also described in Green and Rhodes, "PlantRegeneration in Tissue Culture of Maize," Maize for Biological Research(Plant Molecular Biology Association, Charlottesville, Va. 1982, at367-372) and in Duncan, et al., "The Production of Callus Capable ofPlantRegeneration from Immature Embryos of Numerous Zea Mays Genotypes,"165 Planta 322-332 (1985). Thus, another aspect of this invention is toprovide cells which upon growth and differentiation produce maize plantshaving the physiological and morphological characteristics of inbredline PH02T.

Maize is used as human food, livestock feed, and as raw material inindustry. The food uses of maize, in addition to human consumption ofmaize kernels, include both products of dry- and wet-milling industries.The principal products of maize dry milling are grits, meal and flour.Themaize wet-milling industry can provide maize starch, maize syrups,and dextrose for food use. Maize oil is recovered from maize germ, whichis a by-product of both dry- and wet-milling industries.

Maize, including both grain and non-grain portions of the plant, is alsoused extensively as livestock feed, primarily for beef cattle, dairycattle, hogs, and poultry.

Industrial uses of maize include production of ethanol, maize starch inthewet-milling industry and maize flour in the dry-milling industry. Theindustrial applications of maize starch and flour are based onfunctional properties, such as viscosity, film formation, adhesiveproperties, and ability to suspend particles. The maize starch and flourhave application in the paper and textile industries. Other industrialuses include applications in adhesives, building materials, foundrybinders, laundry starches, explosives, oil-well muds, and other miningapplications.

Plant parts other than the grain of maize are also used in industry: forexample, stalks and husks are made into paper and wallboard and cobs areused for fuel and to make charcoal.

The seed of inbred maize line PH02T, the plant produced from the inbredseed, the hybrid maize plant produced from the crossing of the inbred,hybrid seed, and various parts of the hybrid maize plant can be utilizedfor human food, livestock feed, and as a raw material in industry.

Performance Examples of PH02T

In the examples that follow, the traits and characteristics of inbredmaizeline PH02T are presented as an inbred per se and in hybridcombination. Thedata collected on inbred maize line PH02T is presentedfor key characteristics and traits.

Inbred Comparisons

The results in Table 2A compare inbred PH02T to inbred PHR03. Bothinbreds demonstrate higher than average yields. Both inbreds show muchhigher thanaverage pollen weight, with inbred PH02T demonstrating ahigher pollen weight than inbred PHR03. Both inbreds show very goodpollen scores, with inbred PH02T demonstrating a higher score thanPHR03. Both inbreds show above average tassel size, with inbred PH02Texhibiting larger tassels than inbred PHR03. Inbred PH02T has asignificantly higher stay green score than inbred PHR03.

The results in Table 2B compare inbred PH02T to inbred PH56C. InbredPH02T demonstrates significantly higher yields than inbred PH56C. InbredPH02T shows significantly better seedling vigor than PH56C. Both inbredsshow much higher than average pollen weight. Both inbreds show very goodpollenscores, with inbred PH02T demonstrating a higher score than PH56C.Both inbreds show above average tassel size. Inbred PH02T shows asignificantlyhigher stay green score than inbred PH56C. Inbred PH02Tshows significantlybetter resistance to Gray Leaf Spot than inbredPH56C.

The results in Table 2C compare inbred PH02T to inbred PHBE2. InbredPH02T demonstrates higher yields than inbred PHBE2. Inbred PH02T showssignificantly better seedling vigor than PH56C. Both inbreds show muchhigher than average pollen weight. Both inbreds show very good pollenscores and above average tassel size. Inbred PH02T shows a significantlyhigher stay green score than inbred PHBE2. Inbred PH02T showssignificantly better resistance to Gray Leaf Spot than inbred PHBE2.

Inbred by Tester Comparisons

The results in Table 3A compare the inbred PH02T and inbred PHBE2 wheneachinbred is crossed to the same tester lines. Both the PH02T and thePHBE2 hybrids have above average yields and high test weights. Both thePH02T and the PHR03 hybrids have above average resistance to rootlodging. The PH02T hybrids have above average resistance to stalklodging and have better resistance to stalk lodging than the PHBE2hybrids. The PH02T hybrids demonstrate above average stay green and havea better stay green score than the PHBE2 hybrids. The PH02T hybrids havevery good resistance to Gray Leaf Spot and have better resistance toGray Leaf Spot than the PHBE2 hybrids.

The results in Table 3B compare the inbred PH02T and inbred PHBG4 wheneachinbred is crossed to the same tester lines. The PH02T hybrids showabove average yields and have higher yields than the PHBG4 hybrids. ThePH02T hybrids show significantly lower harvest moisture than the PHBG4hybrids. Both hybrids exhibit high test weights. Both hybridsdemonstrate better than average resistance to root lodging and stalklodging, with the PH02T hybrids having better resistance to stalklodging than the PHBG4 hybrids. Both hybrids demonstrate above averagestay green, with the PH02T hybrids showing better stay green scores thanthe PHBG4 hybrids.

The results in Table 3C compare the inbred PH02T and inbred PH56C wheneachinbred is crossed to the same tester lines. Both the PH02T and thePH56C hybrids have above average yields and high test weights. The PH02Thybridshave above average resistance to root lodging and betterresistance to rootlodging than the PH56C hybrids. Both of the hybridshave above average resistance to stalk lodging and brittle stalk. ThePH02T hybrids have verygood resistance to Gray Leaf Spot and havesignificantly better resistance to Gray Leaf Spot than the PH56Chybrids.

The results in Table 3D compare the inbred PH02T and inbred PHR03 wheneachinbred is crossed to the same tester lines. While the PH02T hybridshave just slightly below above average yields, the PH02T hybrids haveabove average and better resistance to root lodging than the PHR03hybrids. Bothof the hybrids show above average resistance to stalklodging and brittle stalk. Both hybrids demonstrate higher than averagestay green scores and the PH02T hybrids have better stay green scoresthan the PHR03 hybrids.

Hybrid Comparisons

The results in Table 4A compare inbred PH42B crossed to PH02T and inbredPH42B crossed to PH06N. The results show that the PH42BIPH02T hybrid hasabove average yields and a higher yield than the PH42B/PH06N hybrid.Both hybrids show high test weights. The PH42B/PH02T hybrid shows asignificantly higher stay green score than the PH42B/PH06N hybrid. Bothhybrids demonstrate above average resistance to stalk lodging. ThePH42B/PH02T hybrid shows a significantly better resistance to Gray LeafSpot than the PH42B/PH06N hybrid.

The results in Table 4B compare inbred PH42B crossed to PH02T and inbredPH42B crossed to PHN46. Both hybrids show above average yields and hightest weights. The PH42B/PH02T hybrid has a significantly higher staygreenscore than the PH42B/PHN46 hybrid. Both hybrids demonstrate anabove average resistance to root lodging and the PH42B/PH02T hybrid hasan aboveaverage and better resistance to stalk lodging than thePH428/PHN46 hybrid.The PH42B/PH02T hybrid exhibits a very goodresistance to Gray Leaf Spot and a better resistance to Gray Leaf Spotthan the PH42B/PHN46 hybrid.

The results in Table 4C compare inbred PH42B crossed to PH02T and inbredPH42B crossed to PH24E. Both hybrids show above average yields and hightest weights. The results show that the PH42B/PH02T hybrid has an aboveaverage and higher stay green score than the PH42B/PH24E hybrid. Bothhybrids show above average resistance to stalk lodging. The PH42B/PH02Thybrid exhibits a significantly better resistance to Gray Leaf Spot thanthe PH42B/PH24E hybrid.

The results in Table 4D compare inbred PH08B crossed to PH02T and inbredPH08B crossed to PH56C. Both hybrids show slightly below average yieldsbut the PH08B/PH02T hybrid demonstrates better test weights than thePH08B/PH56C hybrid. Both hybrids exhibit above average stay greenscores. Both hybrids demonstrate above average resistance to rootlodging, with the PH08B/PH02T hybrid showing a significantly higherresistance to root lodging than the PH08B/PH56C hybrid. Both hybridsshow above average resistance to stalk lodging. The PH08B/PH02T hybridshows better resistance to Gray Leaf Spot than the PH08B/PH56C hybrid.

                                      TABLE 2A    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT    VARIETY #1 = PH02T    VARIETY #2 = PHR03    __________________________________________________________________________               BU BU     TST                            SDG                               EST                                  TIL                                     GDU                                        GDU                                           POL                                              POL POL                                                     TAS                                                        PLT                                                           EAR RT               ACR                  ACR MST                         WT VGR                               CNT                                  LER                                     SHD                                        SLK                                           WT WT  SC SZ HT HT  LDG               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS                                           ABS                                              % MN                                                  ABS                                                     ABS                                                        ABS                                                           ABS ABS    __________________________________________________________________________    TOTAL SUM           1   98.7                  132 20.6                         51.8                            5.9                               35.6                                  2.0                                     157.2                                        158.9                                           272.2                                              163 7.4                                                     6.5                                                        83.6                                                           32.3                                                               94.6           2   96.2                  127 19.4                         58.1                            5.4                               32.6                                  0.7                                     153.5                                        157.7                                           259.9                                              145 7.1                                                     6.3                                                        85.9                                                           35.5                                                               91.3           LOCS               14 14  18 13 47 71 60 88 86 6  6   13 39 36 30  5           REPS               27 27  31 26 56 82 71 93 90 6  6   13 40 47 40  7           DIFF               2.5                  5   1.2                         0.3                            0.4                               3.0                                  1.3                                     3.7                                        1.2                                           12.3                                              19  0.3                                                     0.2                                                        2.3                                                           3.2 3.2           PR > T               .575                  .491                      .037+                         .423                            .023+                               .015+                                  .003#                                     .000#                                        .031+                                           .898                                              .736                                                  .047+                                                     .202                                                        .004#                                                           .000#                                                               .394    __________________________________________________________________________               STA                  STK                     BRT                        GRN                           SCT                              EAR                                 TEX                                    EAR                                       BAR                                          DRP                                             GLF                                                NLF                                                   SLF                                                      STW                                                         ANT                                                            HD               GRN                  LDG                     STK                        APP                           GRN                              SZ EAR                                    MLD                                       PLT                                          EAR                                             SPT                                                BLT                                                   BLT                                                      WLT                                                         ROT                                                            SMT                                                               CLN               ABS                  ABS                     ABS                        ABS                           ABS                              ABS                                 ABS                                    ABS                                       ABS                                          ABS                                             ABS                                                ABS                                                   ABS                                                      ABS                                                         ABS                                                            ABS                                                               ABS    __________________________________________________________________________    TOTAL SUM           1   6.7                  94.5                     95.9                        7.0                           6.9                              7.3                                 7.0                                    6.3                                       97.0                                          100.0                                             6.0                                                3.5                                                   4.4                                                      6.7                                                         4.4                                                            82.4                                                               5.0           2   4.4                  85.4                     97.2                        7.9                           6.6                              7.1                                 6.8                                    6.7                                       97.4                                          99.3                                             4.6                                                4.3                                                   4.1                                                      6.5                                                         3.9                                                            81.4                                                               5.5           LOCS               32 8  12 5  10 11 11 10 30 3  22 10 8  5  7  4  1           REPS               41 16 15 10 11 11 11 11 32 6  34 19 14 7  12 12 2           DIFF               2.3                  9.3                     1.3                        0.9                           0.3                              0.2                                 0.2                                    0.4                                       0.4                                          0.7                                             1.4                                                0.8                                                   0.3                                                      0.2                                                         0.4                                                            1.0                                                               0.5           PR > T               .000#                  .158                     .609                        .195                           .394                              .553                                 .441                                    .399                                       .776                                          .423                                             .000#                                                .041+                                                   .459                                                      .740                                                         .341                                                            .878    __________________________________________________________________________                                                MDM FUS                                                       DIP                                                          COM                                                             HC ECB                                                CPX ERS                                                       ERS                                                          RST                                                             BLT                                                                1LF                                                ABS ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________                                     TOTAL SUM                                            1   3.6 4.7                                                       2.5                                                          6.0                                                             5.0                                                                4.0                                            2   3.6 6.8                                                       3.0                                                          4.0                                                             6.0                                                                6.6                                            LOCS                                                4   10 1  2  1  3                                            REPS                                                7   12 2  3  1  3                                            DIFF                                                0.0 2.1                                                       0.5                                                          2.0                                                             1.0                                                                2.6                                            PR > T                                                .999                                                    .012+ .000# .122    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 2B    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT    VARIETY #1 = PH02T    VARIETY #2 = PH56C    __________________________________________________________________________               BU BU     TST                            SDG                               EST                                  TIL                                     GDU                                        GDU                                           POL                                              POL POL                                                     TAS                                                        PLT                                                           EAR RT               ACR                  ACR MST                         WT VGR                               CNT                                  LER                                     SHD                                        SLK                                           WT WT  SC SZ HT HT  LDG               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS                                           ABS                                              % MN                                                  ABS                                                     ABS                                                        ABS                                                           ABS ABS    __________________________________________________________________________    TOTAL SUM           1   87.9                  136 21.5                         56.9                            5.9                               35.0                                  1.8                                     157.9                                        159.8                                           240.0                                              159 7.5                                                     6.5                                                        84.4                                                           32.9                                                               97.9           2   56.3                  80  19.3                         56.2                            4.8                               28.9                                  1.8                                     154.5                                        158.8                                           294.2                                              179 7.0                                                     6.2                                                        86.6                                                           34.4                                                               95.9           LOCS               16 16  20 15 42 67 53 76 74 4  4   12 37 32 27  4           REPS               26 26  30 25 51 98 63 81 78 4  4   13 38 41 36  5           DIFF               31.7                  56  2.2                         0.8                            1.1                               6.2                                  0.0                                     3.4                                        1.0                                           54.2                                              20  0.5                                                     0.3                                                        2.3                                                           1.5 2.0           PR > T               .000#                  .000#                      .005#                         .102                            .000#                               .000#                                  .999                                     .000#                                        .077*                                           .577                                              .714                                                  .152                                                     .125                                                        .037+                                                           .143                                                               .601    __________________________________________________________________________               STA                  STK                     BRT                        GRN                           SCT                              EAR                                 TEX                                    EAR                                       BAR                                          DRP                                             GLF                                                NLF                                                   SLF                                                      STW                                                         ANT                                                            HD               GRN                  LDG                     STK                        APP                           GRN                              SZ EAR                                    MLD                                       PLT                                          EAR                                             SPT                                                BLT                                                   BLT                                                      WLT                                                         ROT                                                            SMT                                                               CLN               ABS                  ABS                     ABS                        ABS                           ABS                              ABS                                 ABS                                    ABS                                       ABS                                          ABS                                             ABS                                                ABS                                                   ABS                                                      ABS                                                         ABS                                                            ABS                                                               ABS    __________________________________________________________________________    TOTAL SUM           1   6.8                  94.9                     96.7                        6.6                           6.8                              7.3                                 7.0                                    6.7                                       96.8                                          99.6                                             5.9                                                3.3                                                   4.2                                                      6.6                                                         4.8                                                            83.7                                                               5.0           2   4.8                  90.2                     87.0                        6.6                           6.2                              6.6                                 6.4                                    5.9                                       96.1                                          99.4                                             4.3                                                6.3                                                   5.5                                                      6.6                                                         3.5                                                            89.6                                                               3.0           LOCS               28 11 11 9  13 12 12 12 28 5  19 7  7  4  6  2  1           REPS               35 18 14 14 14 12 12 13 29 8  29 13 12 5  9  6  2           DIFF               2.0                  4.7                     9.7                        0.0                           0.6                              0.7                                 0.6                                    0.8                                       0.8                                          0.2                                             1.6                                                3.0                                                   1.3                                                      0.0                                                         1.3                                                            5.9                                                               2.0           PR > T               .000#                  .155                     .064*                        .999                           .088*                              .087*                                 .002#                                    .241                                       .642                                          .818                                             .000#                                                .000#                                                   .009#                                                      .999                                                         .098*                                                            .158    __________________________________________________________________________                                                MDM FUS                                                       DIP                                                          COM                                                             HC ECB                                                CPX ERS                                                       ERS                                                          RST                                                             BLT                                                                1LF                                                ABS ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________                                     TOTAL SUM                                            1   3.8 4.5                                                       2.5                                                          6.0                                                             5.0                                                                4.0                                            2   2.5 4.1                                                       2.5                                                          4.0                                                             5.0                                                                7.0                                            LOCS                                                2   7  1  2  1  1                                            REPS                                                3   9  2  3  1  1                                            DIFF                                                1.3 0.4                                                       0.0                                                          2.0                                                             0.0                                                                3.0                                            PR > T                                                .126                                                    .607  .000#    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 2C    __________________________________________________________________________    PAIRED INBRED COMPARISON REPORT    VARIETY #1 = PH02T    VARIETY #2 = PHBE2    __________________________________________________________________________               BU BU     TST                            SDG                               EST                                  TIL                                     GDU                                        GDU                                           POL                                              POL POL                                                     TAS                                                        PLT EAR RT               ACR                  ACR MST                         WT VGR                               CNT                                  LER                                     SHD                                        SLK                                           WT WT  SC SZ HT  HT  LDG               ABS                  % MN                      ABS                         ABS                            ABS                               ABS                                  ABS                                     ABS                                        ABS                                           ABS                                              % MN                                                  ABS                                                     ABS                                                        ABS ABS ABS    __________________________________________________________________________    TOTAL SUM           1   66.1                  146 25.6                         53.8                            6.1                               26.2                                  2.1                                     158.3                                        159.8                                           345.3                                              204 7.3                                                     6.5                                                        83.2                                                            32.5                                                                95.8           2   44.8                  94  21.1                         54.1                            4.9                               22.5                                  5.1                                     148.1                                        150.0                                           258.8                                              159 7.4                                                     6.7                                                        81.0                                                            32.2                                                                92.1           LOCS               5  5   5  4  38 53 37 70 69 3  3   12 38 28  22  2           REPS               5  5   5  4  39 72 38 72 70 3  3   12 39 30  24  3           DIFF               21.3                  52  4.5                         0.3                            1.2                               3.7                                  3.0                                     10.2                                        9.8                                           86.5                                              45  0.1                                                     0.2                                                        2.1 0.3 3.6           PR > T               .087*                  .070*                      .061*                         .751                            .000#                               .000#                                  .031+                                     .000#                                        .000#                                           .326                                              .371                                                  .754                                                     .366                                                        .065*                                                            .731                                                                .500    __________________________________________________________________________               STA                  STK                     BRT                        GRN                           SCT                              EAR                                 TEX                                    EAR                                       BAR                                          DRP                                             GLF                                                NLF                                                   SLF                                                      STW                                                         ANT                                                            HD               GRN                  LDG                     STK                        APP                           GRN                              SZ EAR                                    MLD                                       PLT                                          EAR                                             SPT                                                BLT                                                   BLT                                                      WLT                                                         ROT                                                            SMT CLN               ABS                  ABS                     ABS                        ABS                           ABS                              ABS                                 ABS                                    ABS                                       ABS                                          ABS                                             ABS                                                ABS                                                   ABS                                                      ABS                                                         ABS                                                            ABS ABS    __________________________________________________________________________    TOTAL SUM           1   6.5                  94.9                     94.3                        6.0                           6.8                              7.3                                 7.0                                    6.7                                       96.6                                          98.9                                             6.0                                                3.3                                                   4.3                                                      6.7                                                         4.6                                                            76.6                                                                5.0           2   3.5                  89.5                     88.4                        7.0                           7.2                              6.3                                 6.3                                    6.2                                       96.7                                          100.0                                             3.9                                                4.8                                                   2.9                                                      6.9                                                         2.7                                                            99.6                                                                5.0           LOCS               23 3  10 4  13 12 12 12 20 2  23 11 9  5  9  3   1           REPS               24 3  11 4  14 12 12 13 20 2  38 22 17 7  15 10  2           DIFF               3.0                  5.5                     5.9                        1.0                           0.3                              1.0                                 0.8                                    0.5                                       0.1                                          1.1                                             2.1                                                1.5                                                   1.4                                                      0.2                                                         1.9                                                            23.1                                                                0.0           PR > T               .000#                  .194                     .147                        .423                           .121                              .007#                                 .002#                                    .275                                       .953                                          .500                                             .000#                                                .002#                                                   .001#                                                      .621                                                         .004#                                                            .096*    __________________________________________________________________________                                                MDM FUS                                                       DIP                                                          COM                                                             HC ECB                                                CPX ERS                                                       ERS                                                          RST                                                             BLT                                                                1LF                                                ABS ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________                                     TOTAL SUM                                            1   3.5 4.0                                                       2.5                                                          6.0                                                             5.0                                                                4.0                                            2   4.7 4.8                                                       1.5                                                          5.3                                                             4.0                                                                6.3                                            LOCS                                                5   12 1  2  1  3                                            REPS                                                9   16 2  3  1  3                                            DIFF                                                1.2 0.5                                                       1.0                                                          0.8                                                             1.0                                                                2.3                                            PR > T                                                .042+                                                    .151  .205  .192    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 3A    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH02T To PHBE2 Crossed To    The Same Inbred Testers And Grown In The Same Experiments.    __________________________________________________________________________               SEL    BU BU  PRM    TST                                       SDG EST GDU GDU STK PLT EAR               IND PRM                      ACR                         ACR SHD                                MST WT VGR CNT SHD SLK CNT HT  HT               % MN                   ABS                      ABS                         % MN                             ABS                                % MN                                    ABS                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           REPS               6   14 49 49  4  50  37 18  16  7   7   63  22  22           LOCS               6   14 49 49  4  50  37 18  16  7   7   60  22  22           PH02T               103 119                      164                         105 115                                106 57 109 104 101 101 102 100 101           PHBE2               108 116                      160                         104 113                                98  57 114 102 100 98  99  99  101           DIFF               5   3  4  1   2  8   0  6   2   1   2   4   1   0           PR > T               0.50                   0.01                      0.33                         0.74                             0.15                                0.00                                    0.99                                       0.29                                           0.42                                               0.14                                                   0.05                                                       0.07                                                           0.18                                                               0.99    __________________________________________________________________________                                        RT  STA STK BRT GRN DRP GLF                                        LDG GRN LDG STK APP EAR SPT                                        % MN                                            % MN                                                % MN                                                    % MN                                                        % MN                                                            %                                                                ABS    __________________________________________________________________________                             TOTAL SUM                                    REPS                                        18  17  36  3   11  6   9                                    LOCS                                        15  17  33  3   11  6   9                                    PH02T                                        108 122 103 99  108 100 6                                    PHBE2                                        111 114 98  101 109 99  5                                    DIFF                                        3   8   5   2   1   0   1                                    PR > T                                        0.54                                            0.29                                                0.06                                                    0.42                                                        0.90                                                            0.99                                                                0.09    __________________________________________________________________________    *PR > T values are valid only for comparisons with Locs >= 10.

                                      TABLE 3B    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH02T To PHBG4 Crossed To    The Same Inbred Testers And Grown In The Same Experiments.    __________________________________________________________________________               SEL    BU BU  PRM    TST                                       SDG EST GDU GDU STK PLT EAR               IND PRM                      ACR                         ACR SHD                                MST WT VGR CNT SHD SLK CNT HT  HT               % MN                   ABS                      ABS                         % MN                             ABS                                % MN                                    ABS                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           REPS               35  96 340                         340 59 345 207                                       142 174 123 82  441 223 223           LOCS               35  96 279                         279 59 283 164                                       106 120 69  46  338 160 160           PH02T               100 115                      141                         101 113                                103 57 102 95  101 101 100 99  99           PHBG4               98  116                      138                         100 114                                105 57 97  102 102 101 100 99  99           DIFF               2   1  2  1   1  2   0  4   7   1   0   1   0   0           PR > T               0.19                   0.00                      0.05                         0.14                             0.00                                0.00                                    0.99                                       0.05                                           0.02                                               0.00                                                   0.99                                                       0.31                                                           0.99                                                               0.99    __________________________________________________________________________                            RT  STA STK BRT GRN FUS                                                   COM                                                      SOU                                                         ECB                                                            DRP GLF                            LDG GRN LDG STK APP ERS                                                   RST                                                      RST                                                         2SC                                                            EAR SPT                            % MN                                % MN                                    % MN                                        % MN                                            % MN                                                ABS                                                   ABS                                                      ABS                                                         ABS                                                            %                                                                ABS    __________________________________________________________________________                 TOTAL SUM                        REPS                            115 88  277 16  36  1  1  5  64 88  38                        LOCS                            84  70  201 16  36  1  1  5  37 52  38                        PH02T                            103 115 102 100 102 5  5  5  5  100 5                        PHBG4                            109 110 101 101 105 7  6  6  5  100 5                        DIFF                            6   5   2   1   3   2  1  0  1  0   0                        PR > T                            0.19                                0.13                                    0.03                                        0.08                                            0.35      0.99                                                         0.03                                                            0.99                                                                0.99    __________________________________________________________________________    *PR > T values are valid only for comparisons with Locs >= 10.

                                      TABLE 3C    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH02T To PH564 Crossed To    The Same Inbred Testers And Grown In The Same Experiments.    __________________________________________________________________________               SEL    BU BU  PRM    TST                                       SDG EST GDU GDU STK PLT EAR               IND PRM                      ACR                         ACR SHD                                MST WT VGR CNT SHD SLK CNT HT  HT               % MN                   ABS                      ABS                         % MN                             ABS                                % MN                                    ABS                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           REPS               2   33 149                         149 4  149 46 48  41  6   2   152 57  57           LOCS               2   33 149                         149 4  149 46 48  41  6   2   152 57  57           PH02T               104 119                      165                         104 119                                100 57 99  100 102 102 100 98  98           PHBG4               93  118                      163                         103 118                                99  57 90  101 103 105 100 103 106           DIFF               12  1  2  1   0  1   0  8   1   1   3   0   5   8           PR > T               0.25                   0.02                      0.30                         0.28                             0.88                                0.04                                    0.99                                       0.02                                           0.45                                               0.63                                                   0.50                                                       0.99                                                           0.00                                                               0.00    __________________________________________________________________________                                RT  STA STK BRT GRN SLF                                                       FUS                                                          COM                                                             ECB                                                                GLF                                LDG GRN LDG STK APP BLT                                                       ERS                                                          RST                                                             2SC                                                                SPT                                % MN                                    % MN                                        % MN                                            % MN                                                % MN                                                    ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________                     TOTAL SUM                            REPS                                59  26  106 5   4   1  2  2  2  26                            LOCS                                59  26  106 5   4   1  2  2  2  26                            PH02T                                103 94  101 101 104 5  7  7  2  6                            PHBG4                                95  90  101 103 107 7  7  6  3  4                            DIFF                                8   4   0   2   3   2  0  1  1  1                            PR > T                                0.03                                    0.46                                        0.99                                            0.70                                                0.78   0.99  0.50                                                                0.00    __________________________________________________________________________    *PR > T values are valid only for comparisons with Locs >= 10.

                                      TABLE 3D    __________________________________________________________________________    Average Inbred By Tester Performance Comparing PH02T To PHR03 Crossed To    The Same Inbred Testers And Grown In The Same Experiments.    __________________________________________________________________________               SEL    BU BU  PRM    TST                                       SDG EST GDU GDU STK PLT EAR               IND PRM                      ACR                         ACR SHD                                MST WT VGR CNT SHD SLK CNT HT  HT               % MN                   ABS                      ABS                         % MN                             ABS                                % MN                                    ABS                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           REPS               2   4  22 22  1  31  14 4   5   2   2   32  10  10           LOCS               2   4  22 22  1  27  14 4   5   2   2   28  10  10           PH02T               101 117                      152                         99  114                                108 57 95  97  102 104 100 102 101           PHR03               109 115                      161                         107 114                                103 58 104 102 102 105 99  104 104           DIFF               8   2  10 7   0  5   1  9   5   0   1   0   2   2           PR > T               0.22                   0.02                      0.01                         0.01                             0.99                                0.00                                    0.13                                       0.39                                           0.33                                               0.99                                                   0.50                                                       0.99                                                           0.01                                                               0.11    __________________________________________________________________________                                         RT  STA STK BRT SOU                                                            DRP GLF                                         LDG GRN LDG STK RST                                                            EAR SPT                                         % MN                                             % MN                                                 % MN                                                     % MN                                                         ABS                                                            %                                                                ABS    __________________________________________________________________________                              TOTAL SUM                                     REPS                                         15  8   21  4   1  1   4                                     LOCS                                         11  7   18  2   1  1   4                                     PH02T                                         112 132 105 102 4  101 6                                     PHR03                                         87  113 103 102 5  101 5                                     DIFF                                         26  20  2   1   1  0   1                                     PR > T                                         0.13                                             0.02                                                 0.42                                                     0.50   0.99                                                                0.22    __________________________________________________________________________    *PR > T values are valid only for comparisons with Loc >= 10.

                                      TABLE 4A    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PH42B/PH02T    VARIETY #2 = PH42B/PH06N    __________________________________________________________________________                  PRM                     BU BU      TST                                   SDG EST GDU GDU STK PLT EAR RT               PRM                  SHD                     ACR                        ACR MST WT VGR CNT SHD SLK CNT HT  HT  LDG               ABS                  ABS                     ABS                        % MN                            % MN                                ABS                                   % MN                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           1   113                  114                     157.0                        102 102 57.1                                   96  96  104 104 97  103 99  95           2   108                  107                     151.9                        100 93  57.1                                   102 96  96  96  96  98  95  99           LOCS               19 28 78 78  80  57 68  77  45  30  132 52  51  31           REPS               19 28 88 88  92  62 75  90  50  30  166 54  52  32           DIFF               4  7  5.1                        2   10  0.0                                   7   1   8   7   1   5   4   4           PR > T               .000#                  .000#                     .085#                        .329                            .000#                                .999                                   .015+                                       .667                                           .000#                                               .000#                                                   .152                                                       .000#                                                           .000#                                                               .477    __________________________________________________________________________                             STA STK BRT GRN DRP GLF                                                    NLF                                                       STW                                                          DIP                                                             ECB                                                                ECB                             GRN LDG STK APP EAR SPT                                                    BLT                                                       WLT                                                          ERS                                                             DPE                                                                2SC                             % MN                                 % MN                                     % MN                                         % MN                                             % MN                                                 ABS                                                    ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________                  TOTAL SUM                         1   136 104 95  112 100 5.7                                                    7.0                                                       7.5                                                          4.0                                                             100.0                                                                4.5                         2   95  107 95  87  100 3.6                                                    5.0                                                       4.5                                                          3.0                                                             100.0                                                                5.3                         LOCS                             37  61  13  11  30  14 1  2  1  1  11                         REPS                             42  67  19  11  33  17 2  2  1  1  11                         DIFF                             41  4   1   24  0   2.1                                                    2.0                                                       3.0                                                          1.0                                                             0.0                                                                0.7                         PR > T                             .000#                                 .018+                                     .811                                         .004#                                             .999                                                 .000# .000#    .341    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 4B    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PH42B/PH02T    VARIETY #2 = PH42B/PHN46    __________________________________________________________________________                  PRM                     BU BU      TST                                   SDG EST GDU GDU STK PLT EAR RT               PRM                  SHD                     ACR                        ACR MST WT VGR CNT SHD SLK CNT HT  HT  LDG               ABS                  ABS                     ABS                        % MN                            % MN                                ABS                                   % MN                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           1   112                  114                     153.7                        105 103 57.6                                   111 97  104 104 99  104 100 113           2   106                  111                     150.7                        103 89  57.2                                   100 99  100 99  98  100 103 112           LOCS               11 18 68 68  71  49 38  42  23  17  83  31  30  23           REFS               11 18 74 74  79  55 43  47  26  17  100 33  31  24           DIFF               6  3  3.0                        1   14  0.4                                   11  2   3   5   2   4   3   0           PR > T               .000#                  .000#                     .243                        .578                            .000#                                .110                                   .008#                                       .106                                           .000#                                               .000#                                                   .087*                                                       .001#                                                           .124                                                               .999    __________________________________________________________________________                             STA STK BRT GRN DRP GLF                                                    NLF                                                       STW                                                          SOU                                                             ECB                                                                ECB                             GRN LDG STK APP EAR SPT                                                    BLT                                                       WLT                                                          RST                                                             DPE                                                                2SC                             % MN                                 % MN                                     % MN                                         % MN                                             % MN                                                 ABS                                                    ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________                  TOTAL SUM                         1   145 104 90  113 100 6.0                                                    7.0                                                       7.5                                                          6.0                                                             100.0                                                                4.0                         2   89  100 102 92  100 4.0                                                    7.0                                                       6.5                                                          5.0                                                             100.0                                                                4.4                         LOCS                             17  56  5   8   28  5  1  2  1  1  7                         REPS                             17  61  7   8   30  5  2  2  1  1  7                         DIFF                             56  4   13  21  0   2.0                                                    0.0                                                       1.0                                                          1.0                                                             0.0                                                                0.4                         PR > T                             .008#                                 .035+                                     .123                                         .048+                                             .999                                                 .022+ .500     .573    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 4C    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PH42B/PH02T    VARIETY #2 = PH42B/PH24E    __________________________________________________________________________                  PRM                     BU BU      TST                                   SDG EST GDU GDU STK PLT EAR RT               PRM                  SHD                     ACR                        ACR MST WT VGR CNT SHD SLK CNT HT  HT  LDG               ABS                  ABS                     ABS                        % MN                            % MN                                ABS                                   % MN                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           1   113                  114                     161.9                        103 102 57.0                                   98  97  104 104 97  104 99  99           2   108                  108                     157.3                        102 91  57.2                                   87  98  98  97  98  95  91  101           LOCS               16 25 73 73  75  53 56  69  39  27  112 45  44  21           REPS               16 25 83 83  86  56 63  78  42  27  146 47  45  22           DIFF               5  6  4.6                        1   11  0.2                                   11  1   6   7   1   9   8   2           PR > T               .000#                  .000#                     .156                        .528                            .000#                                .408                                   .004#                                       .295                                           .000#                                               .000#                                                   .290                                                       .000#                                                           .000#                                                               .716    __________________________________________________________________________                       STA STK BRT GRN DRP GLF                                              NLF                                                 STW                                                    DIP                                                       SOU                                                          ECB                                                             ECB                                                                ECB                       GRN LDG STK APP EAR SPT                                              BLT                                                 WLT                                                    ERS                                                       RST                                                          DPE                                                             1LF                                                                2SC                       % MN                           % MN                               % MN                                   % MN                                       % MN                                           ABS                                              ABS                                                 ABS                                                    ABS                                                       ABS                                                          ABS                                                             ABS                                                                ABS    __________________________________________________________________________            TOTAL SUM                   1   133 104 96  108 100 5.5                                              7.0                                                 7.5                                                    4.0                                                       6.0                                                          100.0                                                             5.0                                                                4.8                   2   113 106 102 85  100 3.6                                              6.5                                                 6.0                                                    3.0                                                       5.0                                                          100.0                                                             4.0                                                                5.5                   LOCS                       29  52  12  9   25  11 1  2  1  1  1  1  6                   REPS                       31  57  16  9   27  11 2  2  1  1  1  1  6                   DIFF                       20  2   6   24  0   8  0.5                                                 1.5                                                    1.0                                                       1.0                                                          0.0                                                             1.0                                                                0.7                   PR > T                       .015+                           .371                               .116                                   .003#                                       .999                                           .000# .205           .650    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

                                      TABLE 4D    __________________________________________________________________________    INBREDS IN HYBRID COMBINATION REPORT    VARIETY #1 = PH08B/PH02T    VARIETY #2 = PH08B/PH56C    __________________________________________________________________________                  PRM                     BU BU      TST                                   SDG EST GDU GDU STK PLT EAR RT               PRM                  SHD                     ACR                        ACR MST WT VGW CNT SHD SLK CNT HT  HT  LDG               ABS                  ABS                     ABS                        % MN                            % MN                                ABS                                   % MN                                       % MN                                           % MN                                               % MN                                                   % MN                                                       % MN                                                           %                                                               %    __________________________________________________________________________                                                               MN    TOTAL SUM           1   117                  114                     167.6                        94  107 58.3                                   92  104 101 102 101 98  96  112           2   116                  115                     167.0                        94  105 57.5                                   80  91  103 103 94  104 104 106           LOCS               8  5  30 30  31  17 30  29  11  9   45  19  19  13           REPS               8  5  31 31  32  17 33  31  13  9   50  20  20  13           DIFF               1  1  0.6                        0   2   0.8                                   12  13  1   2   7   6   8   6           PR > T               .056*                  .155                     .901                        .999                            .255                                .013+                                   .007#                                       .000#                                           .022+                                               .075*                                                   .001#                                                       .000#                                                           .001#                                                               .009#    __________________________________________________________________________                               STA STK BRT GRN DRP GLF                                                      MDM FUS                                                             DIP                                                                ECB                               GRN LDG STK APP EAR SPT                                                      CPX ERS                                                             ERS                                                                2SC                               % MN                                   % MN                                       % MN                                           % MN                                               % MN                                                   ABS                                                      ABS ABS                                                             ABS                                                                ABS    __________________________________________________________________________                    TOTAL SUM                           1   135 104 94  119 107 4.8                                                      3.0 6.0                                                             7.0                                                                7.0                           2   128 102 88  109 80  4.0                                                      3.5 8.0                                                             7.0                                                                6.0                           LOCS                               21  22  4   3   4   4  1   1  1  1                           REPS                               22  23  4   3   5   4  2   1  1  1                           DIFF                               7   2   6   10  21  0.8                                                      0.5 2.0                                                             0.0                                                                1.0                           PR > T                               .312                                   .430                                       .576                                           .189                                               .347                                                   .319    __________________________________________________________________________    * = 10% SIG     + = 5% SIG     # = 1% SIG

Deposits

A deposit of the seed of inbred PH02T is and has been maintained byPioneerHi-Bred International, Inc., 700 Capital Square, 400 LocustStreet, Des Moines, Iowa 50309-2340, since prior to the filing date ofthis application. Access to this deposit will be available during thependency of the application to the Commissioner of Patents andTrademarks and person determined by the Commissioner to be entitledthereto upon request.Upon allowance of any claims in the application,the Applicant(s) will makeavailable to the public without restriction adeposit of at least 2500 seeds of inbred PH02T with the American TypeCulture Collection (ATCC), Rockville, Md., 20852. The seeds depositedwith the ATCC will be taken from the same deposit maintained at PioneerHi-Bred and described above. Additionally, Applicant(s) will meet allthe requirements of 37 C.F.R. §1.801-1.809, including providing anindication of the viability of the sample when the deposit is made. Thisdeposit of Inbred Maize Line PH02T will be maintained in the ATCCDepository, which is a public depository, for a period of 30 years, or 5years after the most recent request, or for the enforceable life of thepatent, whichever is longer, and will be replaced if it ever becomesnonviable during that period. Applicant will impose no restrictions onthe availability of the depositedmaterial from the ATCC; however,Applicant has no authority to waive any restrictions imposed by law onthe transfer of biological material or its transportation in commerce.Applicant does not waive any infringement of its rights granted underthis patent or under the Plant Variety ProtectionAct (7 USC 2321 etseq.).

The foregoing invention has been described in detail by way ofillustrationand example for purposes of clarity and understanding.However, it will be obvious that certain changes and modifications suchas single gene modifications and mutations, somoclonal variants, variantindividuals selected from large populations of the plants of the instantinbred and the like may be practiced within the scope of the invention,as limited only by the scope of the appended claims.

What is claimed is:
 1. Seed of maize inbred line designated PH02T,representative samples having been deposited under ATCC Accession No.209915.
 2. A maize plant, or parts thereof, having all the physiologicaland morphological characteristics of inbred line PH02T, representativeseed of said line having been deposited under ATCC accession No. 209915.3. The maize plant of claim 2, wherein said plant is male sterile.
 4. Atissue culture of regenerable cells of a maize plant of inbred linePH02T, wherein the tissue regenerates plants capable of expressing allthe morphological and physiological characteristics of the inbred linePH02T, representative seed of which have been deposited under ATCCAccession No.
 209915. 5. A tissue culture according to claim 4, thecells or protoplasts being from a tissue selected from the groupconsisting of leaves, pollen, embryos, roots, root tips, anthers, silks,flowers, kernels, ears, cobs, husks, and stalks.
 6. A maize plantregenerated from the tissue culture of claim 4, said plant capable ofexpressing all the morphological and physiological characteristics ofinbred line PH02T, representative seed of which have been depositedunder ATCC Accession No.
 209915. 7. A method for producing a firstgeneration (F₁) hybrid maize seed comprising crossing the plant of claim2 with a different inbred parent maize plant and harvesting theresultant first generation (F₁) hybrid maize seed.
 8. The method ofclaim 7 wherein inbred maize plant of claim 2 is the female or maleparent.
 9. An F₁ hybrid seed produced by crossing the inbred maize plantaccording to claim 2 with another, different maize plant.
 10. An F₁hybrid plant, or parts thereof, grown from the seed of claim
 9. 11. Aprocess for producing inbred PH02T comprising:planting a collection ofseed comprising seed of a hybrid, one of whose parents is Inbred PH02Tsaid collection also comprising seed of said inbred; growing plants fromsaid collection of seed; identifying said inbred plants; selecting saidinbred plant; and controlling pollination in a manner which preservesthe homozygosity of said inbred plant.
 12. The process of claim 11wherein said step of identifying said inbred plant comprises:identifyingplants with decreased vigor.
 13. The process of claim 11 wherein saidstep of identifying said inbred plant comprises:identifying seeds orplants with homozygous genotype.